How Microbiome Research Is Done: An Overview of Sequencing Methods

Article summary
  • 16S (bacteria/archaea), 18S (eukaryotes), and ITS (fungi) offer cost-effective, group-specific taxonomic profiling.

  • WGS and metatranscriptomics provide species-level and functional insights across all microbes.

  • WGS and metatranscriptomics deliver richer data but require more resources.

 

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5 minutes

Authors

Mariona Castells Ingla

Publication date

A Guide to Key Sequencing Methods and Their Insights

How Microbiome Research Is Done: An Overview of Sequencing Methods

Microbiome research relies on a range of sequencing techniques to identify which microorganisms are present in a sample—and what they’re doing. While these methods aren’t something most people will need to choose between, understanding how they work can offer valuable context for interpreting microbiome data or simply gaining a deeper appreciation of the science. This post provides a clear overview of the main sequencing approaches used in microbiome studies, from 16S and ITS to Whole Genome Sequencing and metatranscriptomics.

16S rRNA Gene Sequencing: For Bacteria and Archaea

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